一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody（cell polarity）

【A】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
ASIP2011-09-27				EP310Y				WB			IF			IHC			IP
				EPT							100
				1694-1
				rabbit
				m				1%PFA 染まるが核染が強い。 Nonspecific nuclear staining.
				HeLa
alkaline phosphatase2013-07-04								WB			IF			IHC			IP
				Rockland							x100
				200-4135				3%PFA (in PBS)
				rabbit				0.05%saponin
								apical staining
				Caco2

【a】

【A】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
alkaline phosphatase2013-12-04								WB			IF			IHC			IP
				Rockland							x100
				200-4135				3%PFA (in PBS)
				rabbit				0.05%saponin
				MDCK

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
calf alkaline phosphatase2011-08-10								WB			IF			IHC			IP
				Rockland							x100
				2001135				3%PFA (in PBS)
				goat
				マウス小腸
human alkaline phosphatase2011-08-10								WB			IF			IHC			IP
				Rockland							x100
				200401142				3%PFA (in PBS)
				rabbit
				マウス小腸
calf alkaline phosphatase2011-08-10								WB			IF			IHC			IP
				Rockland							x100
				2004135				3%PFA (in PBS)
				rabbit
								マウス小腸ではこれがベスト Best Ab of all alkaline phosphatase Abs for frozen section of mouse small intestine.
				マウス小腸

【a】

【A】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
adaptin beta2014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610381				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine
adaptin gamma2014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610385				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine
AP1802014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610469				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine
adaptin alpha2014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610501				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
β-Actin2011-10-14				AC-15				WB			IF			IHC			IP
				Sigma							x10,000
				A5441				methanol 固定可能 methanol fixation also applicable
				mouse
				Mono、IgG1				【抗原の種、部位】N-terminal peptide
				MDCK cells, Hela-K cells
human AQP12011-08-10				B-11				WB			IF			IHC			IP
				Santa Cruz							x100
				sc25287				3%PFA (in PBS), methanol
				mouse
				m
				マウス腎臓
rat AQP12011-08-10				1				WB			IF			IHC			IP
				Santa Cruz							x100
				sc32738				3%PFA (in PBS), methanol
				mouse
				m
				マウス腎臓
human AQP12011-08-10				C-20				WB			IF			IHC			IP
				Santa Cruz							x100
				sc9879				3%PFA (in PBS)
				goat
				p
				マウス腎臓
human AQP22011-08-10				C-17				WB			IF			IHC			IP
				Santa Cruz							x100
				sc9882				3%PFA (in PBS)
				goat
				p
				マウス腎臓
APN2013-07-04								WB			IF			IHC			IP
				BMA Biomedicals							x100
				T-2015				3%PFA (in PBS)
				rat				0.05%saponin
				IgG2A
				Caco2

【a】

【A】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
APN2013-12-04								WB			IF			IHC			IP
				BMA Biomedicals							x100
				T-2015				3%PFA (in PBS)
				rat				0.05%saponin
				IgG2A
				MDCK

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
mouse CD132011-08-10				aminopeptidase				WB			IF			IHC			IP
				BMA							x100
				T2015				3%PFA (in PBS)
				rat
				p
				マウス小腸・腎臓
mouse CD132011-08-10				aminopeptidase				WB			IF			IHC			IP
				BMA							x100
				T2015				3%PFA (in PBS)
				rat
				p
				マウス小腸

【b】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
BiP(GRP78)2014-02-04								WB			IF			IHC			IP
				ABR							x100
				PA1-014				3%PFA (in PBS)
				rabbit				0.05%saponin
				MDCK

【C】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Crumbs2011-11-26				Cq4				WB			IF			IHC			IP
				Developmental study hybridoma bank
								4％PFA +0.1％Triton
				mouse
				Drosophila embryo, discs, nervous system
E-cadherin2011-09-27				EP700Y				WB			IF			IHC			IP
				EPT							100
				1702-1
				rabbit
				m				蛍光染色不可 Not suitable for IF.
				HeLa
E-cadherin (cytoplasmic domain)2011-09-27				clone 36/E-Cadherin				WB			IF			IHC			IP
				BD							100
				610181
				mouse
				m				1%PFA
				DLD?１
Cdc422011-09-27								WB			IF			IHC			IP
				BD							100
				610929
				mouse
				m				TCAのみOK Visualized only by TCA fix.
				MDCK II，HeLa, DLD-1, EpH4, DLD-1, ニワトリ胚マウス胚のプラコード
Cdc422011-09-27								WB			IF			IHC			IP
				Cytoskeleton							100
				ACD01
				rabbit
				p				弱すぎる Too weak staining.
				MDCK II

【c】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
cathepsin D2014-02-04								WB			IF			IHC			IP
				R&D systems							x100
				AF1029				3%PFA (in PBS)
				rabbit				0.05%saponin
				MDCK

【C】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Cdc422011-09-27								WB			IF			IHC			IP
				Santa Cruz							100
				sc-6083
				goat
				p				蛍光染色不可 Not suitable for IF.
				MDCK II
Cdc422011-09-27								WB			IF			IHC			IP
				Santa Cruz							100
				sc-8401
				mouse
				m				蛍光染色不可 Not suitable for IF.
				MDCK II
Cdc422011-09-27								WB			IF			IHC			IP
				Santa Cruz							100
				sc-87
				rabbit
				p				蛍光染色不可 Not suitable for IF.
				MDCK II
Clara cell secretory protein (CCSP)2012-02-22								WB			IF			IHC			IP
				Seven Hills Bioreagents							x100
				WRAB-3950				3%PFA in 0.1M phosphate buffer (pH7.2)
				rabbit				還流固定
								なじみのない会社なので注文には時間がかかるかもしれません Ordering may take time because this Ab is produced by a small company.
				mouse lung frozen section

【D】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Discs large (Dlg)2011-11-26								WB			IF			IHC			IP
				Developmental study hybridoma bank S
								4％PFA +0.1％Triton
				Mouse
				Drosophila embryo, discs, nervous system
DPPIV2012-04-04				HBB3/775/42 monoclonal				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank							x5
								3%PFA in PBS
				mouse				0.05% saponin
				IgG1				culture sup
				Caco2				JCB 101, 838-851, 1985

【d】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Discs large (Dlg)2014-09-04				4F3				WB			IF			IHC			IP
				DHSB							x50
								4%PFA (in phosphate buffer)
				mouse
				IgG1
				Drosohila imaginal disc

【D】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
mouse DPPIV2011-08-10				CD26 ectodomain				WB			IF			IHC			IP
				R&D Systems							x100
				AF954				3%PFA (in PBS)
				goat
				マウス小腸
DPP IV2013-07-04								WB			IF			IHC			IP
				R&D systems							x100
				AF954				3%PFA (in PBS)
				goat				0.05%saponin
								apical staining
				Caco2

【d】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
DPP IV2013-12-04								WB			IF			IHC			IP
				R&D systems							x100
				AF954				3%PFA (in PBS)
				goat				0.05%saponin
				MDCK

【D】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
human DPPIV2011-08-10				CD26 H-270				WB			IF			IHC			IP
				Santa Cruz							x100
				sc9153				3%PFA (in PBS)
				rabbit
				p
				マウス小腸

【e】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
E-cadherin (dog) 2016-08-10				rr1				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank (DSHB)							x300
								4%PFA (in phosphate buffer)
				Mouse				0.1% TritonX-100/PBS
				IgG1
				MDCK cell
EEA12014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610456				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine
eps152014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610806				3%PFA (in phosphate buffer)
				mouse
				IgG2a
				mouse small intestine

【E】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
EB2 / MAPRE22012-08-23								WB			IF			IHC			IP
				abcam				x500
				ab123579				IF: -20℃ methanol or acetone:methanol=1:1
				rabbit
				HeLa細胞
EB3 / MAPRE32012-08-23								WB			IF			IHC			IP
				abcam				x1,000-2,000			x500-1,000
				ab126953				IF: -20℃ methanol or acetone:methanol=1:1
				rabbit
				HeLa細胞
EB1/MAPRE12012-08-23 About Product				Polyclonal				WB			IF			IHC			IP
				Abcam				x1,000-5,000			x200-1,000
				ab50188
				rabbit				IF: -20℃ methanol or acetone:methanol=1:1
				IgG				チューブリンにも反応するので要注意 Also recognize tubulin
				HeLa細胞
Ezrin2011-10-14				3C12				WB			IF			IHC			IP
				SIGMA				x 1000			x 500
				E8897				2％ PFA 0.5%Triton, RT 15min
				mouse
				Mono、IgG1				【抗原の種、部位】human 362-585 a.a.
				MDCK cells
mouse E-cadherin2011-08-10								WB			IF			IHC			IP
				Takara				x1000			x100
				M108				3%PFA (in PBS)
				rat
				m				通常の染色法で大丈夫 Stains well by ordinary staining procedures.
				マウス小腸、腎臓

【g】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
GS152014-02-04								WB			IF			IHC			IP
				Transduction							x100
				G72420				3%PFA (in PBS)
				mouse				0.05%saponin
				IgG1
				MDCK
GS272014-02-04								WB			IF			IHC			IP
				Transduction							x100
				G76220				3%PFA (in PBS)
				mouse				0.05%saponin
				IgG1
				MDCK

【H】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
CRB32011-09-27								WB			IF			IHC			IP
				Sigma							100
				HPA013835
				rabbit
				p				1%PFA, TCA
				DLD?１

【L】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
bovine LDLR2011-08-10								WB			IF			IHC			IP
				Progen							x100
				61087				3%PFA (in PBS), methanol
				mouse
				m
				マウス小腸
L12011-08-12				8D9				WB			IF			IHC			IP
				DSHB
				8D9
				mouse
								PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
mouse LDLR2011-08-10				NS0				WB			IF			IHC			IP
				R&D Systems				x1000			x100
				AF2255				3%PFA (in PBS)
				goat
				マウス小腸、腎臓
L12011-08-12								WB			IF			IHC			IP
				Chemicon
				MAB5272
				rat
				m				PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
mouse LDLR2011-08-10				M-20				WB			IF			IHC			IP
				Santa Cruz							x100
				sc11826				3%PFA (in PBS), methanol
				goat
				p
				マウス小腸

【M】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
maltase2012-04-04				HBB2/143/17 monoclonal				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank							x5
								3%PFA in PBS
				mouse				0.05% saponin
				IgG1				culture sup
				Caco2				JCB 101, 838-851, 1985
MDR1+32011-08-10				clone:C219				WB			IF			IHC			IP
				Zymed							x100
				18-7244				methanol
				mouse
								メタノール固定でＯＫ Good staining by methanol fix.
				マウス肝臓
MRP22011-08-10				M2?-5)=MDR2				WB			IF			IHC			IP
				Alexis
				ALX-801-037
				mouse
				m

【N】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Na,K-ATPase2011-11-26				a5				WB			IF			IHC			IP
				Developmental study hybridoma bank S
								4％PFA +0.1％Triton
				mouse
				IgG
				Drosophila nervous system
Na,K-ATPase2011-11-26				Nrv5F7				WB			IF			IHC			IP
				Developmental study hybridoma bank S
								4％PFA +0.1％Triton
				mouse
				IgG
				Drosophila nervous system
NaK ATPase αsubunit2012-04-04				α5 monoclonal				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank							x50
								3%PFA in PBS
				mouse				0.05% saponin
				IgG1				concentrated culture sup
				Caco2				EMBO J 8, 193-202
NaK ATPase α1subunit2012-04-04				α6F monoclonal				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank							x50
								3%PFA in PBS
				mouse				0.05% saponin
				IgG2a				concentrated culture sup
				Caco2				JBC 263, 4347-54, 1988
Na+/K+ ATP ase2011-10-14				C464.6				WB			IF			IHC			IP
				Millipore/Upstate							x 300
				05-369				2％ PFA 0.5%Triton, RT 15min, methanol 固定可能 Methanol fixation also applicable
				mouse
				Mono、IgG1				【抗原の種、部位】rabbit whole protein
				MDCK cells

【n】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Na+K+ ATPase2015-01-13								WB			IF			IHC			IP
				Upstate biotechnology							x100
				05-369				3%PFA (in PBS)
				Mouse
				IgG1κ
				mouse kidney frozen section
Na+K+ ATPase2015-02-27								WB			IF			IHC			IP
				Upstate biotechnology							x100
				05-369				3%PFA (in PBS)
				Mouse
				IgG1κ
				mouse kidney frozen section
beta NAP2014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610892				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine

【N】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
NGF-receptor　2011-08-10				p75				WB			IF			IHC			IP
				NeoMarkers							x100
				MS394				3%PFA (in PBS)
				mouse
				m
				マウス小腸

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
PAR-1b/MARK2/EMK 2011-10-14								WB			IF			IHC			IP
PAR-4/LKB2011-10-14								WB			IF			IHC			IP
PAR-6β2011-10-14								WB			IF			IHC			IP
								2％ PFA 0.5%Triton, RT 15min
				MDCK cells
PAR-1a/MARK3/c-TAK1 2011-10-14				183.A10.A3				WB			IF			IHC			IP
				Millipore/Upstate							x 300
				05-680				2％ PFA 0.5%Triton, RT 15min
				mouse
				Mono, unknown				【抗原の種、部位】human full length
				MDCK cells
PAR32011-08-14								WB			IF			IHC			IP
				upstate							x100
				07-330				3%PFA (in PBS)
				rabbit
				p
				マウス小腸
PAR-32011-09-27								WB			IF			IHC			IP
				Millipore (Upstate)							100
				07-330
				rabbit
				p				1%PFA, TCA
				DLD-1, EpH4, MTD-1A
Par32011-10-03								WB			IF			IHC			IP
				upstate							x500
				07-330
				rabbit				【Formalin in PBS+0.05%Triton】:○
				poly				ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
				rat hippocampal neurons
PAR-32011-10-14				―				WB			IF			IHC			IP
				Millipore/Upstate				x 500			x 500
				07-330				2％ PFA 0.5%Triton, RT 15min
				rabbit
				Poly				【抗原の種、部位】mouse 457-744 a.a
				MDCK cells

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
PKD 12014-01-07								WB			IF			IHC			IP
				Cell Signaling Technology				x500			x100
				2052				3%PFA (in PBS)
				rabbit
				IgG				WB: cross reaction with PKD2
				WB: several tissues; IF: Lung
PKD 32014-01-07								WB			IF			IHC			IP
				Cell Signaling Technology				x1000
				5655
				rabbit
				IgG
				WB: several tissues

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
aPKC iota2011-10-14				23/PKCi				WB			IF			IHC			IP
				BD Bioscience				x 1000			x 100
				610176				2％ PFA 0.5%Triton, RT 15min
				mouse
				Mono、IgG2b				【抗原の種、部位】human a.a. 404-587 aPKC iota はllamdaと同じである APKC iota is the same as aPKC lamda
				MDCK cells

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
p47A2014-04-03								WB			IF			IHC			IP
				BD Transduction							x100
				610900				3%PFA (in phosphate buffer)
				mouse
				IgG1
				mouse small intestine

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
human PKD22011-08-10				BL844				WB			IF			IHC			IP
				Bethyl
				A300074A
				rabbit
PKD22011-08-14 About Product				Monoclonal				WB			IF			IHC			IP
				Abcam
				ab51250
				rabbit
				IgG
human podocalyxin2011-08-10								WB			IF			IHC			IP
				R&D Systems							x100
				AF1658				methanol
				goat
								メタノール固定で弱く染まる？ Weak staining by methanol fix.
				MDCK

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
podocalyxin2014-02-04								WB			IF			IHC			IP
				R&D systems							x100
				AF1658				3%PFA (in PBS)
				goat				0.05%saponin
				MDCK

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
human p75 pAb2011-08-10								WB			IF			IHC			IP
				Promega							x100
				G3231				3%PFA (in PBS)
				rabbit
				ｐ				神経らしき線維が染まる Axon-like fibers are detected by this Ab.
				マウス小腸
human p75 pAb2011-08-12								WB			IF			IHC			IP
				Promega
				G3231
				rabbit
				ｐ				PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human p75 pAb2012-08-03								WB			IF			IHC			IP
				Promega							x100
				G3231				3%PFA in PBS
				rabbit				0.05% saponin
				PC12
PAR-5/14-3-3 b/z2011-10-14								WB			IF			IHC			IP
				Stressgen				x 1000
				KAM-CC012
				mouse
				Mono, unknown				【抗原の種、部位】lysed trransformed cells

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
PKD 12014-01-07								WB			IF			IHC			IP
				Sigma				x100000			x100
				P 3987				3%PFA (in PBS)
				rabbit
				IgG				WB: cross reaction with PKD2
				WB: several tissues; IF: Lung

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
human PepT12011-08-10								WB			IF			IHC			IP
				Acris							x100
				R1532P				3%PFA (in PBS), methanol
				rabbit
				p
				マウス小腸
PKCzeta2011-09-27								WB			IF			IHC			IP
				Santa Cruz							100
				sc-216
				rabbit
				p				1%PFA, TCA
				DLD-1, EpH4, MTD-1A

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
PKC zeta (C-20)2014-09-04								WB			IF			IHC			IP
				Santa Cruz							x500
				sc-216				4%PFA (in phosphate buffer)
				rabbit
				Drosohila imaginal disc

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
aPKC (zeta and lambda)2011-10-14				―				WB			IF			IHC			IP
				Santa Cruz				x 1000			x 300
				sc-216（C-20)				2％ PFA 0.5%Triton, RT 15min
				rabbit
				Poly				【抗原の種、部位】rat C-terminus
				MDCK cells
PAR-5/14-3-3 (pan)2011-10-14				―				WB			IF			IHC			IP
				Santa Cruz				x 500
				sc-629 (K-19)				2％ PFA 0.5%Triton, RT 15min
				rabbit
				Poly				【抗原の種、部位】human N-terninal
				MDCK cells

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
PKD 12014-01-07								WB			IF			IHC			IP
				St. Cruz Biotechnology C-20				x100000			x100
				SC-639				3%PFA (in PBS)
				rabbit
				IgG				WB: cross reaction with PKD2
				WB: several tissues; IF: Lung
PKD 12014-01-07								WB			IF			IHC			IP
				St. Cruz Biotechnology D-20				x2000			x100
				SC-935				3%PFA (in PBS)
				rabbit
				IgG				WB: cross reaction with PKD2
				WB: several tissues; IF: Lung

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
human PepT12011-08-10				H-235				WB			IF			IHC			IP
				Santa Cruz							x100
				sc20653				methanol
				rabbit
				p				メタノール固定で染まる、WBはＯＫ Methanol fixation is OK for IF. Also applicable for WB
				マウス小腸

【p】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
PDI2014-02-04								WB			IF			IHC			IP
				StressGen							x100
				SPA891				3%PFA (in PBS)
				mouse				0.05%saponin
				MDCK

【P】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
pro-SPC2012-02-21								WB			IF			IHC			IP
				Seven Hills Bioreagents							x100
				WRAB-9337				3%PFA in 0.1M phosphate buffer (pH7.2)
				rabbit				還流固定
								なじみのない会社なので注文には時間がかかるかもしれません Ordering may take time because this Ab is produced by a small company.
				mouse lung frozen section

【S】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
sucrase-isomaltase2012-04-04				HBB2/614/88 monoclonal				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank							x5
								3%PFA in PBS
				mouse				0.05% saponin
				IgG1				culture sup
				Caco2				JCB 101, 838-851, 1985
sucrase-isomaltase2012-04-04				HBB2/219/20 monoclonal				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank							x5
								3%PFA in PBS
				mouse				0.05% saponin
				IgG2b				culture sup
				Caco2				JCB 101, 838-851, 1985
syntaxin32013-07-04								WB			IF			IHC			IP
				synaptic systems				x1000
				rabbit
								no staining
				mouse brain, intestine
Syntaxin42011-08-14								WB			IF			IHC			IP
				BD transduction
				610439
				mouse
				m
syntaxin32013-07-04								WB			IF			IHC			IP
				chemicon				x1000
				AB5448
				rabbit
								nonspecific band at ca.30k even in KO tissue
				mouse brain, intestine
Sodium Potassium ATPase2011-09-27 About Product				Monoclonal				WB			IF			IHC			IP
				Abcam							200-500
				ab7671
				mouse
				IgG1				4%PFA, 1%PFA, TCA
				DLD?１
syntaxin32013-07-04								WB			IF			IHC			IP
				gene tex				x1000
				GTX24113
				rabbit
								nonspecific band at ca.30k even in KO tissue
				mouse brain, intestine

【s】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
syntaxin32013-12-04								WB			IF			IHC			IP
				gene tex							x100
				GTX24113				3%PFA (in PBS)
				rabbit				0.05%saponin
				MDCK

【S】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
Syntaxin32011-08-14								WB			IF			IHC			IP
				Atlas
				HPA002191				3%PFA (in PBS), methanol
				rabbit
				p
				マウス小腸
syntaxin32013-07-04								WB			IF			IHC			IP
				atlas antibodies (sigma)				x1000
				HPA002191
				rabbit
								no staining
				mouse brain, intestine
syntaxin32013-07-04								WB			IF			IHC			IP
				sigma				x1000
				s5567
				rabbit
								nonspecific band at ca.30k even in KO tissue
				mouse brain, intestine
Scrib2011-09-27								WB			IF			IHC			IP
				Santa Cruz							100
				sc-11049
				goat
				p				4%PFA, 1%PFA, TCA
				DLD?１
SGLT2013-07-04								WB			IF			IHC			IP
				santa cruz							x100
				sc-20581				3%PFA (in PBS)
				goat				0.05%saponin
				Caco2

【s】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
SGLT2013-12-04								WB			IF			IHC			IP
				santa cruz							x100
				sc-20581				3%PFA (in PBS)
				goat				0.05%saponin
				MDCK

【S】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
mouse SGLT-12011-08-10				M-19				WB			IF			IHC			IP
				Santa Cruz							x100
				sc20582				3%PFA (in PBS)
				goat
				p
				マウス小腸

【T】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
TfR2011-09-27				H68.4				WB			IF			IHC			IP
				Zymed				1:1000			1:100
				13-6800
				mouse
				m
				HeLa, A549, MCF7他
human Transferrin Receptor2011-08-12								WB			IF			IHC			IP
				Zymed
				136800
				mouse
								PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human Transferrin Receptor2011-08-10								WB			IF			IHC			IP
				Zymed							x100
				136800				3%PFA (in PBS), methanol
				mouse
				マウス小腸
human Transferrin Receptor2011-08-10								WB			IF			IHC			IP
				Zymed							x100
				136800				3%PFA (in PBS), methanol
				mouse
								MDCKは染まる　３Ｔ３は染まらない Stains MDCK, but not 3T3.
				マウス小腸
Biotin mouse CD712011-08-10				TFR				WB			IF			IHC			IP
				BD Pharmingen							x100
				557416				3%PFA (in PBS), methanol
				rat
				m
				マウス小腸
TfR2011-09-27								WB			IF			IHC			IP
				BD Biosciences				1:1000			1:100
				612125
				mouse
				m				human専用
				HeLa, A549, MCF7他
CD712011-08-10				TFR				WB			IF			IHC			IP
				Zymed							x100
				77103				3%PFA (in PBS), methanol
				マウス小腸
CD712011-08-10				TFR				WB			IF			IHC			IP
				Zymed							x100
				77103				3%PFA (in PBS), methanol
				マウス小腸
CD712011-08-10				R17217 TFR				WB			IF			IHC			IP
				Santa Cruz							x100
				sc52504				3%PFA (in PBS), methanol
				rat
				m
				マウス小腸
CD712011-08-10				R17217 TFR				WB			IF			IHC			IP
				Santa Cruz							x100
				sc52504				3%PFA (in PBS), methanol
				rat
				m
				マウス小腸

【U】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
human Unc18-1/22011-08-10				N-19				WB			IF			IHC			IP
				Santa Cruz				x1000
				sc14560
				goat
				p
				マウス
human Unc18-22011-08-10				C-20				WB			IF			IHC			IP
				Santa Cruz				x1000
				sc14563
				goat
				p
				マウス
utrophin2011-10-14				―				WB			IF			IHC			IP
				Santa Cruz				x 1,000			x 1,000
				UT-2				2％ PFA 0.5%Triton, RT 17min
				rabbit
				Poly				【抗原の種、部位】human 1768-2078 a.a.
				MDCK cells

【V】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
mVAMP72011-08-10				0H307				WB			IF			IHC			IP
				IBL
				2930
				rabbit
TI-VAMP protein2011-08-10				VAMP 7				WB			IF			IHC			IP
				Novus B							x100
				NB1002325				3%PFA (in PBS), methanol
				mouse
				m
				マウス小腸
TI-VAMP protein2011-08-10				VAMP 7				WB			IF			IHC			IP
				Novus B							x100
				NB1002325				3%PFA (in PBS), methanol
				mouse
				m
				マウス小腸
VAMP72011-08-10								WB			IF			IHC			IP
				Abnova							x100
				PAB5936				3%PFA (in PBS), methanol
				rabbit
				p
				マウス小腸
human Villin2011-08-10				C-19				WB			IF			IHC			IP
				Santa Cruz							x100
				sc7672				3%PFA (in PBS)
				goat
				p				染色薄い Weak staining
				マウス小腸
vinculin2011-10-14				hVIN-1				WB			IF			IHC			IP
				Sigma							x 500
				V9131				2％ PFA 0.5%Triton, RT 15min, methanol 固定可能 Methanol fixation also applicable
				mouse
				Mono、IgG1				【抗原の種、部位】human whole protein
				MDCK cells

【z】

Antigen last update				Clone				Application outlook for label
				Maker				Dilution rate
				Catalog code				Fixation condition for IF and IHC
				Host				Fixation and permeabilization condition
				Isotype				Comments
				Samples				Reference
ZO-1 (Mouse)2016-08-10				R26.4C				WB			IF			IHC			IP
				Developmental Studies Hybridoma Bank (DSHB)							x300
								4%PFA (in phosphate buffer)
				Rat				0.1% TritonX-100/PBS
				IgG1
				MDCK cell